🧬✨ Mitochondrial Transcription: A Click-Chemistry Derived Detection Method Without Radiation
Mitochondrial transcription plays a crucial role in cellular energy production, as mitochondria contain their own DNA and transcriptional machinery. Traditionally, studying mitochondrial RNA synthesis in vitro relied on radioactive labeling techniques, which posed safety concerns, disposal challenges, and regulatory limitations. ⚠️🧪 Recent advances now offer a safer and more efficient alternative through click-chemistry–based detection methods, eliminating the need for hazardous radioactive isotopes while maintaining high sensitivity and accuracy. 🔬💡 Click chemistry enables the incorporation of modified nucleotides into newly synthesized mitochondrial RNA. These modified molecules contain bioorthogonal functional groups that can react selectively with fluorescent probes under mild conditions. The result is a rapid, highly specific labeling process that allows researchers to visualize and quantify transcription products using fluorescence instead of radiation. This approach enhances ...
