🔬 Validating DNA Reference Material with dPCR

The creation of a linearized plasmid DNA certified reference material (CRM) ,

marks a major step forward in ensuring accuracy and consistency in genetic measurement science 🔬. By designing a highly stable DNA construct with traceable quantification, researchers aim to support labs worldwide in achieving reliable calibration and quality control for molecular assays.

To guarantee precision, scientists employed single-molecule direct counting and digital PCR,

two cutting-edge, ultra-sensitive quantification methods . These techniques enable absolute measurement without the need for standard curves, reducing uncertainty and enhancing reproducibility. The linearized DNA CRM was carefully optimized to maintain integrity, purity, and sequence accuracy across diverse analytical platforms.

An extensive interlaboratory validation involving multiple expert labs confirmed the CRM,

 robustness, repeatability, and cross-platform compatibility . This collaborative effort ensures that laboratories globally can adopt the reference material with confidence, supporting advancements in diagnostics, biotechnology, and regulatory science. Ultimately, this work strengthens trust in genetic testing and accelerates innovation in molecular biology .
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