🧪🔬 Comprehensive Chemical Profiling of Wenjing Decoction via 2D-LC-HRMS & GC-MS

 Wenjing decoction, a classic traditional Chinese medicine formula, is widely used for gynecological and circulatory disorders. Due to its complex herbal composition, comprehensive chemical characterization is essential to understand its therapeutic basis. Advanced analytical strategies now enable deeper insights into its multi-component nature.


In this study, offline two-dimensional liquid chromatography coupled with high-resolution mass spectrometry (2D-LC-HRMS) was employed to achieve enhanced separation and accurate identification of polar and semi-polar constituents. Complementary gas chromatography–mass spectrometry (GC-MS) was used to profile volatile and low-molecular-weight compounds, ensuring broad chemical coverage.

📊 The integrated LC-HRMS and GC-MS approach allowed systematic identification of diverse bioactive compounds, including flavonoids, organic acids, alkaloids, and volatile oils. This comprehensive analytical framework not only improves quality control of Wenjing decoction but also supports pharmacological research and modernization of traditional herbal medicines.

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