⚗️ State-of-the-Art Monolithic Chromatography for Large Biomolecules

 Monolithic chromatography has emerged as a powerful separation technique for biological nanoparticles and large biomolecules such as proteins, viruses, plasmid DNA, and extracellular vesicles. 🧪 Unlike traditional packed columns, monolithic supports feature a continuous porous structure that allows high permeability and low backpressure 🚀, enabling fast mass transfer and efficient separation of large, fragile biomolecules without structural damage.



Recent developments have focused on advanced polymer- and silica-based monolithic materials with tailored pore sizes and surface chemistries . These innovations enhance binding capacity, selectivity, and biocompatibility, making them ideal for high-resolution purification in biopharmaceutical and nanobiotechnology applications . Functionalization strategies, including ion-exchange, affinity, and hydrophobic interactions, further expand their versatility in handling complex biological samples .

State-of-the-art monolithic chromatography is now widely integrated into downstream processing workflows for vaccines, gene therapy vectors, and nanomedicine formulations . Its scalability, compatibility with high-throughput processing, and reduced solvent consumption align well with green and sustainable analytical practices . As research advances, monolithic supports are expected to play a crucial role in next-generation bioseparation technologies ✨.

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